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Image Search Results
Journal: Scientific Reports
Article Title: A systems-approach reveals human nestin is an endothelial-enriched, angiogenesis-independent intermediate filament protein
doi: 10.1038/s41598-018-32859-4
Figure Lengend Snippet: EC nestin is regulated by laminar shear stress in vitro . Immunofluorescence staining of nestin in HUVEC, HDMEC, HCAEC and HPAEC cultured under ( A ) static conditions, or ( B ) 10 dyne/cm 2 laminar shear stress for 24 hours. Quantification of nestin spatial distribution in ( C ) HUVEC, ( D ) HDMEC, ( E ) HCAEC and ( F ) HPAEC. Each point represents an individual cell (n = 3–19 different experiments) Unpaired t-test *p-value < 0.05, **<0.01 ****<0.0001. ( G ) HUVEC were cultured under 10 dyne/cm 2 laminar shear stress for 0 h, 1 h, 4 h or 24 h, and stained for nestin, vimentin, actin, and DAPI. Pearson’s co-localisation value for nestin and vimentin is displayed on the top right of the merged images. ( H ) Quantification of nestin spatial distribution over the time course (n = 6); significance was calculated using one-way ANOVA. ( I ) NES and ( J ) VIM mRNA expression in EC under both static conditions and laminar shear stress, displayed relative to 18 s rRNA (n = 6–10). All graphs show means ± SD.
Article Snippet: Human Pulmonary Artery Endothelial Cells (HPAEC), Human Coronary Artery Endothelial Cells (HCAEC), and Human Dermal Microvascular Endothelial Cells (HDMEC) were obtained from Promocell in cryogenically frozen vials, and were cultured in EC Growth Medium (HPAEC, HCAEC) or
Techniques: In Vitro, Immunofluorescence, Staining, Cell Culture, Expressing